initiator protein造句

• Iterons complex with cognate replication ( Rep ) initiator proteins to achieve the required regulatory effect.
• First, the initiator protein makes another nick to terminate synthesis of the first ( leading ) strand.
• This structure consists of an origin of replication upstream of a gene that codes for a replication initiator protein.
• The open reading frame encodes a rolling circle replication initiator protein ( Rep ) similar to that found in the nanoviruses.
• In prokaryotic DNA replication regulation focuses on the binding of the DnaA initiator protein to the DNA, with initiation of replication occurring multiple times during one cell cycle.
• In both geminiviruses and nanoviruses this sequence contains the origin of replication ( " ori " ) and is nicked by the rolling circle replication initiator protein to initiate viral DNA replication.
• Rolling circle DNA replication is initiated by an initiator protein encoded by the plasmid or bacteriophage DNA, which nicks one strand of the double-stranded, circular DNA molecule at a site called the double-strand origin, or DSO . The initiator protein remains bound to the 5'phosphate end of the nicked strand, and the free 3'hydroxyl end is released to serve as a primer for DNA synthesis by DNA polymerase III . Using the unnicked strand as a template, replication proceeds around the circular DNA molecule, displacing the nicked strand as single-stranded DNA . Displacement of the nicked strand is carried out by a host-encoded helicase called PcrA ( the abbreviation standing for plasmid copy reduced ) in the presence of the plasmid replication initiation protein.
• Rolling circle DNA replication is initiated by an initiator protein encoded by the plasmid or bacteriophage DNA, which nicks one strand of the double-stranded, circular DNA molecule at a site called the double-strand origin, or DSO . The initiator protein remains bound to the 5'phosphate end of the nicked strand, and the free 3'hydroxyl end is released to serve as a primer for DNA synthesis by DNA polymerase III . Using the unnicked strand as a template, replication proceeds around the circular DNA molecule, displacing the nicked strand as single-stranded DNA . Displacement of the nicked strand is carried out by a host-encoded helicase called PcrA ( the abbreviation standing for plasmid copy reduced ) in the presence of the plasmid replication initiation protein.